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Evaluation of a Chemical Method for Assay of Helminthosporium maydis Race T Toxin
Author(s) -
O. C. Yoder,
Ver Gracen
Publication year - 1977
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.59.5.792
Subject(s) - toxin , race (biology) , biology , host (biology) , bioassay , microbiology and biotechnology , botany , genetics
A chemical test reported by Karr et al. (Plant Physiol. 55:727) to assay for host-specific toxin produced by Helminthosporium maydis race T was evaluated. Preparations from culture filtrates of both race T isolates, containing host-specific toxin, and similar preparations from race O isolates, containing no detectable host-specific toxin, gave positive reactions in the chemical assay. Also, preparations containing active or inactive toxin gave equal responses in this test. The procedure does not provide a reliable method for assaying H. maydis race T toxin.

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