Alpha-Ketoglutarate Supply for Amino Acid Synthesis in Higher Plant Chloroplasts | Zendy

Bin A. Elias | Zendy; Curtis V. Givan | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Alpha-Ketoglutarate Supply for Amino Acid Synthesis in Higher Plant Chloroplasts

Author(s) -

Bin A. Elias,

Curtis V. Givan

Publication year - 1977

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.59.4.738

Subject(s) - chloroplast , biochemistry , isocitrate dehydrogenase , dehydrogenase , biology , glutamate dehydrogenase , microbody , pisum , chlorophyll , peroxisome , photorespiration , chloroplast membrane , photosynthesis , enzyme , botany , glutamate receptor , thylakoid , gene , receptor

Isocitrate dehydrogenase was found in Pisum sativum chloroplasts purified on sucrose density gradients. A chloroplast-enriched pellet obtained by differential centrifugation formed two chlorophyll-containing bands. The lower one containing intact chloroplasts had NADP-specific isocitrate dehydrogenase and triose-phosphate isomerase activities. Mitochondria and peroxisomes were observed to band well away from the intact chloroplast region, as indicated by peak activities of fumarase and catalase, respectively. The presence of isocitrate dehydrogenase in chloroplasts suggests that chloroplasts may generate at least some of the alpha-ketoglutarate required for glutamate synthesis.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research