Growth of Suspension-cultured Acer pseudoplatanus L. Cells in Automatic Culture Units of Large Volume | Zendy

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Growth of Suspension-cultured Acer pseudoplatanus L. Cells in Automatic Culture Units of Large Volume

Author(s) -

Richard Bligny

Publication year - 1977

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.59.3.502

Subject(s) - acer pseudoplatanus , chemostat , suspension (topology) , suspension culture , botany , biology , cell culture , tissue culture , plant cell , liquid culture , volume (thermodynamics) , chromatography , chemistry , horticulture , biochemistry , in vitro , genetics , mathematics , physics , quantum mechanics , homotopy , bacteria , gene , pure mathematics

A phytostat to mass culture higher plant cells in liquid medium is described. This apparatus allowed the culture in batch, turbidostat and chemostat of 20 liters of cells. Automatic control of cell suspension growth was based on culture turbidity. Changes with time of certain cell characteristics, particularly cell respiration and phospholipid content, indicated that the test time to harvest large amounts of sycamore cells (Acer pseudoplatanus L.) in good physiological state was about 2 days before the end of the exponential phase of growth, when the cell density reached one million cells per milliliter of culture.

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