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Light-induced De-epoxidation in Lettuce Chloroplasts
Author(s) -
D. Siefermann,
Harry Y. Yamamoto
Publication year - 1976
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.57.6.939
Subject(s) - violaxanthin , thylakoid , stroma , chloroplast , biology , membrane , chloroplast stroma , chlorophyll , photosynthesis , chemistry , zeaxanthin , botany , biophysics , biochemistry , carotenoid , lutein , immunohistochemistry , gene , immunology
Grana and stroma lamellae fractions prepared from illuminated chloroplasts (Lactuca sativa L. var. Manoa) by French press treatment contained less violaxanthin and more zeaxanthin than the corresponding fractions from dark controls. In both fractions, only part of the total violaxanthin was de-epoxidized under illumination, and the ratio of de-epoxidized and unchanged violaxanthin was similar. This not only shows that the de-epoxidation system is present in both grana and stroma thylakoids but also that violaxanthin is heterogeneous in both membranes. The presence and similarity of the de-epoxidation system in grana and stroma lamellae suggest that the function of the violaxanthin cycle is linked to photosynthetic activities which are common to both types of membranes.

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