Auxin Biogenesis | Zendy

Peter Bower | Zendy; Hugh M. Brown | Zendy; William K. Purves | Zendy

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Auxin Biogenesis

Author(s) -

Peter Bower,

Hugh M. Brown,

William K. Purves

Publication year - 1976

Publication title -

plant physiology

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.57.6.850

Subject(s) - hypocotyl , cotyledon , auxin , biochemistry , cucumis , cell fractionation , biogenesis , biology , reductase , cytosol , subcellular localization , microsome , enzyme , microbiology and biotechnology , botany , cytoplasm , gene

Subcellular fractionation of cucumber (Cucumis sativus L.) seedlings was achieved, and two of the enzymes in the auxin biosynthetic pathway were localized. NADH-specific indoleacetaldehyde reductase activity was observed only in the cytosol fractions obtained from separated hypocotyl and cotyledon tissue. In contrast, a portion of the NADPH-specific indoleacetaldehyde reductase activity was associated with a microsomal fraction derived from these tissues. The NADPH-specific indoleacetaldehyde reductase was consistently found to be more firmly associated with the microsomal fraction derived from hypocotyls than with that from the cotyledons. These results indicate a division of the terminal steps of auxin biogenesis into at least two subcellular compartments.

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