Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L.
Author(s) -
Kunihiko Syōno,
John G. Torrey
Publication year - 1976
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.57.4.602
Subject(s) - sativum , pisum , cytokinin , zeatin , biology , riboside , callus , botany , horticulture , biochemistry , auxin , gene
Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea (Pisum sativum L., cv. Little Marvel). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems. Smaller activities of zeatin ribotide, isopentenyladenine and its riboside were also detected. Cytokinin activity gradually decreased with the cultivation period, but no qualitative change in the active compounds was found.
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