Open AccessIsotope Discrimination by Ribulose 1,5-Diphosphate Carboxylase
Author(s) -
John T. Christeller,
William A. Laing,
John H. Troughton
Publication year - 1976
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.57.4.580
Subject(s) - ribulose , bicarbonate , pyruvate carboxylase , glycine , rubisco , chemistry , biochemistry , isotope , isotopes of carbon , botany , enzyme , biology , amino acid , environmental chemistry , organic chemistry , physics , quantum mechanics , total organic carbon
Carbon 13 isotope discrimination by ribulose 1,5-diphosphate carboxylase from soybean (Glycine max [Merr.] cv. Amsoy) was studied as a function of temperature, bicarbonate concentration, and pH. None of these factors affected the degree of discrimination against (13)C. The average delta(13)C was -28.3%, a value close to that found for whole C(3) plants. The zero temperature response observed here with ribulose 1,5-diphosphate carboxylase corroborates data from whole plants. The lack of effect of bicarbonate concentration on discrimination is consistent with both current theories of alternate forms of carboxylase.