Indoleacetic Acid Synthesis in Soybean Cotyledon Callus Tissue | Zendy

Robert C. Black | Zendy; Robert H. Hamilton | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Indoleacetic Acid Synthesis in Soybean Cotyledon Callus Tissue

Author(s) -

Robert C. Black,

Robert H. Hamilton

Publication year - 1976

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.57.3.437

Subject(s) - cotyledon , tryptophan , callus , anthranilic acid , auxin , glycine , tryptamine , biochemistry , tissue culture , chemistry , indole test , botany , biology , amino acid , in vitro , gene

Growth of an auxin-requiring soybean cotyledon callus tissue (Glycine max L., Merr. var. Acme) was promoted by tryptophan, tryptamine, indole, indoleacetamide and, to a very slight degree, anthranilic acid. When tryptophan-3-(14)C was supplied in the growth medium, labeled indoleacetic acid (IAA) was found in both the tissue and the medium. Medium, from which the cells had been removed, was also found to convert labeled tryptophan to IAA. Soybean callus contained 0.044 mumole/g free tryptophan, but this is apparently not available for conversion to IAA. These results suggest that while exogenously supplied trytophan could elevate a specific internal pool where IAA synthesis occurs some of the growth on a tryptophan medium can be accounted for by external conversion.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research