Influence of the Nutrient Medium on the Recovery of Dividing Cells from Tobacco Protoplasts | Zendy

Hirofumi Uchimiya | Zendy; Toshio Murashige | Zendy

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Influence of the Nutrient Medium on the Recovery of Dividing Cells from Tobacco Protoplasts

Author(s) -

Hirofumi Uchimiya,

Toshio Murashige

Publication year - 1976

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.57.3.424

Subject(s) - protoplast , nutrient , biology , botany , chemistry , microbiology and biotechnology , ecology

Systematic tests resulted in a nutrient solution containing the following, in milligrams per liter, for the culture of protoplasts isolated from Nicotiana tabacum L. callus cells: Murashige and Skoog salts (T. Murashige and F. Skoog, 1962. Physiol. Plant. 15: 473-497); sucrose, 15,000; mannitol, 110,000; alpha-naphthaleneacetic acid, 0.6; kinetin, 0-0.1; thiamine.HCl, 10; pyridoxine.HCl, 10; nicotinic acid, 5; myo-inositol, 100; and glycine, 2. In this medium, regeneration of cell wall has been observed in 85% and resumption of cell division among 35% of the protoplast isolates.

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