Nonmetabolic Catalysis of 14C-Furfuryl Amino Purine (Kinetin) on Plant Surfaces, Glass, and Porcelain Ware | Zendy

Roy M. Sachs | Zendy; Kay Ryugo | Zendy; Olav Messerschmidt | Zendy

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Nonmetabolic Catalysis of ¹⁴C-Furfuryl Amino Purine (Kinetin) on Plant Surfaces, Glass, and Porcelain Ware

Author(s) -

Roy M. Sachs,

Kay Ryugo,

Olav Messerschmidt

Publication year - 1976

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.57.1.98

Subject(s) - kinetin , degradation (telecommunications) , chemistry , chromatography , laboratory flask , botany , organic chemistry , biochemistry , biology , tissue culture , telecommunications , computer science , in vitro

Kinetin-8-(14)C degraded rapidly upon drying on living or inert surfaces. However, when care was exercised to avoid taking solutions to dryness during fractionation of plant extracts containing (14)C-kinetin and before partitioning by thin layer chromatography, little degradation occurred. A procedure for 24-hour ethyl acetate partitioning, using a continuous liquid-liquid extractor, which permits nearly complete removal of kinetin from aqueous solutions, is herein described. High natural light intensities in the greenhouse or from fluorescent/incandescent sources greatly enhanced nonmetabolic degradation of kinetin on leaf (Bougainvillea) or glass surfaces, which indicated that this may be a confounding factor in analyzing metabolism of kinetin in plant tissues. One of the degradation products is probably adenine.

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