Isolation and Characterization of the Multiple 7S Globulins of Soybean Proteins | Zendy

Vu Huu Thanh | Zendy; Kazuyoshi Ōkubo | Zendy; Kazuo Shibasaki | Zendy

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Isolation and Characterization of the Multiple 7S Globulins of Soybean Proteins

Author(s) -

Vu Huu Thanh,

Kazuyoshi Ōkubo,

Kazuo Shibasaki

Publication year - 1975

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.56.1.19

Subject(s) - sephadex , chromatography , globulin , hydroxymethyl , glycine , tris , chemistry , electrophoresis , ionic strength , fraction (chemistry) , soybean proteins , biochemistry , enzyme , biology , amino acid , stereochemistry , organic chemistry , soy protein , aqueous solution , immunology

Two major proteins (the 7S and 11S globulins) of soybean (Glycine max) were simultaneously isolated by a simple method based on their different solubilities in dilute tris (hydroxymethyl) aminomethane buffers. The purified 7S globulins, which represented essentially the entire 7S soybean protein fraction capable of dimerization at 0.1 ionic strength, were fractionated into five components by diethylaminoethyl Sephadex A-50 column chromatography. The five 7S components were characterized by disc-electrophoresis.

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