Morphological and Photosynthetic Properties of Digitonin-treated Chloroplast Membranes from the Wild-type and ac-5 Strains of Chlamydomonas reinhardi | Zendy

Katherine E. Steinback | Zendy; Ursula Goodenough | Zendy

Open Access

Morphological and Photosynthetic Properties of Digitonin-treated Chloroplast Membranes from the Wild-type and ac-5 Strains of Chlamydomonas reinhardi

Author(s) -

Katherine E. Steinback,

Ursula Goodenough

Publication year - 1975

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.55.5.864

Subject(s) - digitonin , chloroplast , membrane , chlamydomonas , photosystem , chloroplast membrane , mixotroph , biology , photosystem ii , fractionation , photosystem i , photosynthesis , cell fractionation , biophysics , chemistry , thylakoid , biochemistry , chromatography , bacteria , genetics , mutant , gene , heterotroph

Chloroplast membranes of wild-type Chlamydomonas reinhardi, treated with digitonin, yield photosystem II-rich and photosystem I-rich fractions; this fractionation is accompanied by a separation of stacked (grana) lamella from unstacked (stroma) lamellae. Poor fractionation of the photosystems occurs when the treated chloroplast membranes derive from the ac-5 strain grown mixotrophically, whereas good fractionation occurs with ac-5 cells grown phototrophically; the mixotrophic cells possess only unstacked membranes, whereas the phototrophic cells possess stacked membranes. We concluded that digitonin fractionation is dependent on the stacked membrane configuration.

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