Open AccessPartial Reactions of Photosynthesis in Briefly Sonicated Chlamydomonas
Author(s) -
Virginia A. Curtis,
Jerry J. Brand,
Robert K. Togasaki
Publication year - 1975
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.55.2.183
Subject(s) - sonication , chlamydomonas , chloroplast , photosynthesis , breakage , chemistry , biophysics , botany , biology , materials science , biochemistry , chromatography , mutant , composite material , gene
The cell structure of Chlamydomonas reinhardi is disrupted by brief exposure to sonication. The extent of cell breakage can be determined quickly by cell count with the light microscope. Rates of photochemical activities of briefly sonicated cells approach those reported for higher plant chloroplasts. These activities are a sensitive function of time of sonication and sonic power used. The method of brief sonication is rapid and convenient and gives a stable preparation useful for determining photochemical activities in Chlamydomonas.