Open AccessEndogenous Levels of Auxin and Tumorigenesis in a Nicotiana Amphiploid
Author(s) -
Ira H. Ames
Publication year - 1974
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.54.6.953
Subject(s) - endogeny , auxin , indole 3 acetic acid , nicotiana , acetic acid , botany , solanaceae , carcinogenesis , biology , chemistry , biochemistry , horticulture , gene
Young seedlings of the tumor-prone amphiploid Nicotiana suaveolens X N. langsdorffii were grown aseptically on nutrient medium in a controlled environment chamber. At regular intervals after the seeds had been sown, the incidence of tumor formation was scored and plants were harvested. Indole-3-acetic acid was extracted, purified, and assayed spectrofluorometrically. A close correlation in time between onset of tumor formation and decline in endogenous level of indole-3-acetic acid was demonstrated.