Open AccessRole of Free Space in Translocation in Sugar Beet
Author(s) -
Donald R. Geiger,
Susan A. Sovonick,
Terri L. Shock,
Robert J. Fellows
Publication year - 1974
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.54.6.892
Subject(s) - phloem , sucrose , mannitol , photosynthesis , sugar , sugar beet , chromosomal translocation , chemistry , free space , botany , plasmolysis , symplast , biology , biophysics , biochemistry , apoplast , horticulture , cell wall , physics , gene , optics
The involvement of the free space in phloem loading of sucrose was studied in sugar beet source leaves (Beta vulgaris, L.). Sucrose, supplied exogenously to the abraded upper surface of leaves at a concentration of 20 mm, was available for translocation at rates similar to those obtained with photosynthesis. The exogenous sucrose substituted as a source of translocate for assimilate derived from photosynthesis when the latter process was disrupted by plasmolysis of the leaf with 0.8 M mannitol. The mesophyll symplast was not completely disrupted by this treatment, however. Data from the sugar uptake experiments indicate that phloem loading can occur from the free space.Isotope trapping of labeled sugars derived from (14)CO(2) was used to intercept and identify sugars passing through the free space prior to phloem loading. Increased translocation rates induced by 4 mm ATP or increased light intensity were accompanied by increased trapping of sucrose but not of glucose. The data support the view that sucrose passes into the free space prior to phloem loading.