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Concentrations of Indole-3-acetic Acid and Its Esters in Avena and Zea
Author(s) -
Robert S. Bandurski,
Aga Schulze
Publication year - 1974
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.54.3.257
Subject(s) - avena , acetic acid , indole 3 acetic acid , chemistry , hydrolysis , indole test , alkaline hydrolysis , zea mays , organic chemistry , biochemistry , botany , agronomy , biology , auxin , gene
An isotope-dilution method has been developed for the assay of free indole-3-acetic acid and ester indole-3-acetic acid as measured by indole-3-acetic acid liberated by mild alkaline hydrolysis. Application of this method to seedlings of Avena sativa and Zea mays indicates the upper limit of free indole-3-acetic acid in Avena to be about 16 mug per kg and in Zea, about 24 mug. The amount of 1 n alkali-labile indole-3-acetic acid in Zea is about 330 mug per kg and there is very little 1 n alkali-labile IAA in Avena. A chemical characterization of the indole-3-acetic acid of Avena and a confirmation of the chemical characterization of the indole-3-acetic acid of Zea is presented.

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