Open AccessDistribution of Ribosomal Deoxyribonucleic Acid in Subcellular Fractions of Higher Plants
Author(s) -
Adam Jaworski,
Joe L. Key
Publication year - 1974
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.53.3.366
Subject(s) - ribosomal rna , dna , ribosomal dna , biology , chemistry , biochemistry , botany , gene , phylogenetics
Methods are described by which ribosomal DNA can be enriched in subcellular fractions of carrot and soybean. With both carrot and cucumber it was possible to obtain a distinct satellite DNA which contained the rDNA. Hybridization values greater than 0.49% were necessary before a satellite component was observed. Saturation hybridization values for soybean, carrot, and cucumber DNA were 0.2, 0.49, and 1.14%, respectively. These values were increased 1.6- and 2-fold in soybean and carrot, respectively, but enrichment was not obtained for cucumber.