A Polygalacturonase from Citrus Leaf Explants

The relationship between polygalacturonase activity and abscission of citrus leaf explants was studied. Determination of polygalacturonase activity in citrus tissues requires concentration of the enzyme, use of a proper assay method, and inhibition of an oxidase present in the extracts which oxidizes the reaction products of the polygalacturonase. The polygalacturonase from citrus leaf explants is an exopolygalacturonase and appears to be a soluble enzyme.Polygalacturonase activity increased during abscission of citrus leaf explants and was localized in the separation layer. Ethylene accelerated the increase in polygalacturonase activity, but its effect was evident only after at least an 8-hour lag period. 2,4-Dichlorophenoxyacetic acid and cycloheximide inhibited abscission and polygalacturonase activity. It is concluded that polygalacturonase, in addition to cellulase, plays a role in abscission.