Formation of Adenosine 5′-Phosphorofluoridate and the Assay of Adenyl Cyclase in Barley Seeds | Zendy

Robert Alvarez | Zendy; Thomas C. Moore | Zendy; Jozef Vandepeute | Zendy

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Formation of Adenosine 5′-Phosphorofluoridate and the Assay of Adenyl Cyclase in Barley Seeds

Author(s) -

Robert Alvarez,

Thomas C. Moore,

Jozef Vandepeute

Publication year - 1974

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.53.2.144

Subject(s) - cyclase , hordeum vulgare , sodium fluoride , adenosine , incubation , enzyme , chemistry , biochemistry , adenosine monophosphate , adenylate kinase , endogeny , hordeum , fluoride , biology , poaceae , botany , inorganic chemistry

Barley seed (Hordeum vulgare L.) homogenates contain an apparent enzymatic activity which catalyzes the synthesis of adenosine 5'-phosphorofluoridate from magnesium-adenosine 5'-triphosphate and sodium fluoride. Formation of this compound may interfere with some adenyl cyclase assays which use fluoride as a component of the incubation medium. Neither adenyl cyclase activity nor endogenous adenosine 3': 5'-monophosphate was detected in barley seed homogenates or extracts.

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