The Oxidation of Malate and Exogenous Reduced Nicotinamide Adenine Dinucleotide by Isolated Plant Mitochondria | Zendy

David A. Day | Zendy; Joseph T. Wiskich | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The Oxidation of Malate and Exogenous Reduced Nicotinamide Adenine Dinucleotide by Isolated Plant Mitochondria

Author(s) -

David A. Day,

Joseph T. Wiskich

Publication year - 1974

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.53.1.104

Subject(s) - antimycin a , nad+ kinase , rotenone , biochemistry , mitochondrion , malate dehydrogenase , nicotinamide adenine dinucleotide , malonate , chemistry , biology , enzyme

Exogenous NADH oxidation by cauliflower (Brassica oleracea L.) bud mitochondria was sensitive to antimycin A and gave ADP/O ratios of 1.4 to 1.9. In intact mitochondria, NADH-cytochrome c reductase activity was only slightly inhibited by antimycin A. The antimycin-insensitive activity was associated with the outer membrane. Malate oxidation was sensitive to both rotenone and antimycin A and gave ADP/O values of 2.4 to 2.9. However in the presence of added NAD(+), malate oxidation displayed similar properties to exogenous NADH oxidation. In both the presence and absence of added NAD(+), malate oxidation was dependent on inorganic phosphate and inhibited by 2-n-butyl malonate.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research