Separation and Ultrastructure of Proplastids from Dark-grown Euglena Cells | Zendy

I. Ophir | Zendy; Yehuda BenShaul | Zendy

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Separation and Ultrastructure of Proplastids from Dark-grown Euglena Cells

Author(s) -

I. Ophir,

Yehuda BenShaul

Publication year - 1973

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.51.6.1109

Subject(s) - ultrastructure , euglena , biology , microbiology and biotechnology , botany , euglena gracilis , chloroplast , biochemistry , gene

A procedure for the separation of proplastids free of mitochondria from dark-grown Euglena cells has been developed. A fraction enriched in proplastids was used for freeze-etching study of proplastid structure. The prolamellar body in freeze-etched replicas appeared sponge-like, with thylakoids, often vesicular, emerging from it. The prolamellar body and the thylakoids were covered by particles of about 100A in diameter. No larger particles, typical of light-grown chloroplasts, were observed.

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