Characterization of Deoxyribonucleic Acid Species from Castor Bean Endosperm | Zendy

Stephen A. Douglass | Zendy; Richard S. Criddle | Zendy; R. W. Breidenbach | Zendy

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Characterization of Deoxyribonucleic Acid Species from Castor Bean Endosperm

Author(s) -

Stephen A. Douglass,

Richard S. Criddle,

R. W. Breidenbach

Publication year - 1973

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.51.5.902

Subject(s) - endosperm , dna , biology , biochemistry , nuclear dna , rna , biogenesis , buoyant density , escherichia coli , sucrose , dna polymerase , botany , mitochondrial dna , gene

Three DNA buoyant density species (nuclear, 1.692 g cm(-3); mitochondria 1.705 g cm(-3); and proplastid, 1.713 g cm(-3)) can be detected in extracts from castor bean endosperm. No other buoyant density species can be identified. DNA extracts from sucrose density gradient purified glyoxysomes exhibit varying amounts of each of the three identified DNAs but no other distinguishable DNA species. RNA synthesized in vitro by Escherichia coli RNA polymerase using purified castor bean nuclear DNA as a template, hybridizes equally well with its template and with the 1.692 g cm(-3) species from glyoxysome fractions. These results are discussed in terms of their relevance to microbody biogenesis.

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