Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa | Zendy

Robert H. Schloemer | Zendy; Reginald H. Garrett | Zendy

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Partial Purification of the NADH-Nitrate Reductase Complex from Chlorella pyrenoidosa

Author(s) -

Robert H. Schloemer,

Reginald H. Garrett

Publication year - 1973

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.51.3.591

Subject(s) - chlorella pyrenoidosa , nitrate reductase , nitrate , chlorella , chemistry , ammonium , biochemistry , ammonium sulfate , reductase , enzyme , polyethylene glycol , michaelis–menten kinetics , chromatography , fractionation , organic chemistry , biology , enzyme assay , botany , algae

The nitrate reductase complex from Chlorella pyrenoidosa has been purified by a procedure which includes as main steps, ammonium sulfate fractionation, polyethylene glycol treatment, and DEAE-cellulose chromatography. The Michaelis constants for NADH, FAD, and NO(3) (-) in the NADH-nitrate reductase assay are 10 mum, 2.6 mum, and 0.23 mm, respectively. Heat treatment exerts varying effects on the enzymatic activities associated with the nitrate reductase complex.

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