Open AccessPossible Errors in Quantitative Determination of Phenylalanine Ammonia-Lyase Activity by Spectrophotometric Methods
Author(s) -
A. Erez
Publication year - 1973
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.51.2.409
Subject(s) - phenylalanine , phenylalanine ammonia lyase , ammonia , chemistry , lyase , chromatography , biochemistry , enzyme , amino acid
A possible error in spectrophotometric determination of cinnamate, the product of phenylalanine ammonia-lyase activity, using nonpurified protein extracts has been shown.Under optimal conditions for phenylalanine ammonia-lyase activity, with borate buffer and in the presence of alpha-keto acids, phenylpyruvate is produced and its enol tautomer-borate complex formed, strongly absorbs at 290 nanometers.
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