Detergent Extraction of Enzymes from Tobacco Leaves Varying in Maturity | Zendy

Donald W. De Jong | Zendy

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Detergent Extraction of Enzymes from Tobacco Leaves Varying in Maturity

Author(s) -

Donald W. De Jong

Publication year - 1972

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.50.6.733

Subject(s) - peroxidase , acid phosphatase , chlorogenic acid , biology , isozyme , polyphenol oxidase , enzyme , malate dehydrogenase , botany , oxidoreductase , tobacco leaf , protease , biochemistry , chemistry , horticulture , agricultural engineering , engineering

Enzyme activities of tobacco leaves were compared in detergent extracts. Highest levels of chlorogenic acid oxidase, malate-NAD oxidoreductase, and acid phosphatase were obtained from bud tissues. Peroxidase activity was least in young leaves and highest in senescent leaves yellowed with Ethrel. Peroxidase zymograms obtained by means of disc electrophoresis showed differences in isozyme composition among all five samples examined. Although protease was found in material extracted from buds, upper, middle, and lower leaf positions, none could be demonstrated in Ethrel-treated mature leaves.

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