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Soybean seedlings were examined for the presence of mitochondrial tRNA. Tyrosyl transfer tRNAs from whole cells, from a well characterized mitochondrial preparation, and from a snake venom phosphodiesterase-treated mitochondrial preparation, were compared by reverse phase chromatography. It was concluded that none of the three previously reported tRNA(Tyr) species were mitochondrial. Rather, a fourth tRNA(Tyr) species, eluting somewhat later, was of mitochondrial origin. Mitochondrial tRNA(Tyr) was chromatographically similar to Escherichia coli tRNA(Tyr).

Mitochondrial Tyrosyl Transfer Ribonucleic Acid in Soybean Seedlings