Open AccessCharacterization of Short Time Labeled Adenosine Monophosphate-rich Ribonucleic Acids of Soybean
Author(s) -
Joe L. Key,
Christopher J. Leaver,
Joe R. Cowles,
James Michael Anderson
Publication year - 1972
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.49.5.783
Subject(s) - fractionation , rna , chromatography , adenosine , polysome , chemistry , mole , acrylamide , albumin , sucrose , biochemistry , salt (chemistry) , nucleic acid , specific activity , population , enzyme , ribosome , organic chemistry , demography , sociology , copolymer , gene , polymer
The total population of newly synthesized (32)P-AMP-rich RNA has been separated into two major types based on repeated fractionation on methylated albumin-kieselguhr columns. The purified D-RNA which elutes, under our experimental conditions, primarily in the salt gradient has a GMP/AMP ratio of about 0.8 and an AMP + UMP content of about 56 mole per cent. The purified TB-RNA which preferentially remains bound to the column in the salt gradient has a GMP/AMP ratio of about 0.4 to 0.45 and an AMP + UMP content of about 65 mole per cent. In addition to being distinguished by their fractionation on the methylated albumin-kieselguhr column and base composition analysis, purified D-RNA and TB-RNA have different size distributions on sucrose gradient and acrylamide gel fractionation, are differentially associated with polyribosomes and have different stabilities in the tissue.
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