Indoleacetic Acid Biosynthesis in Avena Coleoptile Tips and Excised Bean Shoots | Zendy

Robert C. Black | Zendy; Robert H. Hamilton | Zendy

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Indoleacetic Acid Biosynthesis in Avena Coleoptile Tips and Excised Bean Shoots

Author(s) -

Robert C. Black,

Robert H. Hamilton

Publication year - 1971

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.48.5.603

Subject(s) - coleoptile , tryptophan , avena , indole test , anthranilic acid , elongation , biochemistry , tryptamine , shoot , biology , biosynthesis , indole 3 acetic acid , chemistry , botany , amino acid , auxin , enzyme , materials science , gene , metallurgy , ultimate tensile strength

Avena coleoptiles did not elongate when incubated with tryptophan under sterile conditions. Indole, anthranilic acid, and tryptamine promoted elongation. Under the same conditions, the tissue converted tryptophan-(14)C to IAA-(14)C. More IAA-(14)C was produced from indole-(14)C than from tryptophan-(14)C; however, the free tryptophan content of the tissue was also greatly increased by the indole treatment. Tryptophan-(14)C was readily taken up by the tissue but was mainly incorporated into protein and did not increase the free tryptophan level. When bean shoots were labeled with tryptophan-(14)C or indole-(14)C, the label incorporation into IAA-(14)C was very nearly the same. In this tissue the free tryptophan level in the tryptophan-(14)C and indole-(14)C treatments was also about equal. These results suggest that failure of exogenously supplied tryptophan to promote the elongation of Avena coleoptiles is a result of its predominant incorporation into protein and consequent unavailability for conversion to IAA.

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