Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation | Zendy

Aurea C. Vasconcelos | Zendy; Marilyn S. Pollack | Zendy; Leticia Mendiola | Zendy; HansPeter Hoffmann | Zendy; D. H. Brown | Zendy; C. Price | Zendy

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Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation

Author(s) -

Aurea C. Vasconcelos,

Marilyn S. Pollack,

Leticia Mendiola,

HansPeter Hoffmann,

D. H. Brown,

C. Price

Publication year - 1971

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.47.2.217

Subject(s) - euglena gracilis , chloroplast , euglena , centrifugation , ribosome , ficoll , differential centrifugation , rubisco , ultrastructure , biochemistry , biology , rna , chemistry , botany , photosynthesis , peripheral blood mononuclear cell , gene , in vitro

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

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