The Fractionation of Transfer Ribonucleic Acid from Roots of Pea Seedlings | Zendy

Larry N. Vanderhoef | Zendy; Joe L. Key | Zendy

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The Fractionation of Transfer Ribonucleic Acid from Roots of Pea Seedlings

Author(s) -

Larry N. Vanderhoef,

Joe L. Key

Publication year - 1970

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.46.2.294

Subject(s) - transfer rna , pisum , phenylalanine , biology , biochemistry , methionine , rna , serine , ribonuclease , sativum , proline , amino acid , leucine , valine , threonine , botany , enzyme , gene

Isoaccepting transfer RNA species for several amino acids were fractionated by reverse phase column chromatography. Transfer RNA from dividing cells of pea (Pisum sativum) root was compared to that from nondividing cells, and no relative quantitative or qualitative differences were noted for the transfer RNA species for leucine, lysine, proline, threonine, methionine, serine, and phenylalanine. However, certain artifactual differences for serine and phenylalanine were noted. Quantitative differences were observed in tyrosyl-transfer RNA's. Ribonuclease action on tRNA did not contribute to the tRNA species observed.

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