Some Spectral Properties of Pea Phytochrome in Vivo and in Vitro | Zendy

Marylee S. Everett | Zendy; Winslow R. Briggs | Zendy

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Some Spectral Properties of Pea Phytochrome in Vivo and in Vitro

Author(s) -

Marylee S. Everett,

Winslow R. Briggs

Publication year - 1970

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.45.6.679

Subject(s) - phytochrome , in vivo , in vitro , phytochrome a , pigment , biophysics , spectral line , biology , botany , chemistry , physics , biochemistry , red light , genetics , arabidopsis thaliana , gene , organic chemistry , astronomy , mutant

The transformation difference spectrum for phytochrome (Pr spectrum minus Pfr spectrum) in pea tissue is determined below 560 nanometers and compared with similar data on phytochrome in vitro The difference spectrum in vivo between phytochrome intermediates and Pfr is also shown for comparison with the data on phytochrome solutions. These comparisons show that the peaks in the spectra occurring in the blue wave lengths are shifted to shorter wave lengths and are much enhanced when phytochrome is extracted from the cell and placed in solution. The results indicate that the physicochemical state of phytochrome in the cell may be different from that of the extracted pigment.

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