Gas-Liquid Chromatographic Isolation of Cytokinins from Natural Sources | Zendy

Christen D. Upper | Zendy; John P. Helgeson | Zendy; John D. Kemp | Zendy; C. J. Schmidt | Zendy

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Gas-Liquid Chromatographic Isolation of Cytokinins from Natural Sources

Author(s) -

Christen D. Upper,

John P. Helgeson,

John D. Kemp,

C. J. Schmidt

Publication year - 1970

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.45.5.543

Subject(s) - purine , chromatography , cytokinin , agrobacterium tumefaciens , yeast , hydrolysate , extraction (chemistry) , chemistry , isolation (microbiology) , gas chromatography , biology , biochemistry , hydrolysis , enzyme , microbiology and biotechnology , auxin , transgene , gene

Gas-liquid chromatographic retention times for 20 purines or purine nucleosides, 14 of which are highly active cytokinins, are reported. With one exception, all of the naturally occurring cytokinins are separated.Ethyl acetate extraction of yeast transfer RNA hydrolysates and of culture filtrates of Agrobacterium tumefaciens gave sufficient concentration of the naturally occurring cytokinins for immediate gas-liquid chromatography. This procedure permitted the detection of 6-(3-methyl-2-butenylamino)-9-beta, d-ribofuranosylpurine in yeast transfer RNA extracts. An active cytokinin was isolated from A. tumefaciens culture filtrates and was tentatively identified as 6-(3-methyl-2-butenylamino)purine.

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