The Use of Dimethylsulfoxide as a Solvent in the Tobacco Bioassay for Cytokinins | Zendy

Ruth Y. Schmitz | Zendy; Folke Skoog | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The Use of Dimethylsulfoxide as a Solvent in the Tobacco Bioassay for Cytokinins

Author(s) -

Ruth Y. Schmitz,

Folke Skoog

Publication year - 1970

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.45.4.537

Subject(s) - bioassay , solvent , chemistry , botany , biology , toxicology , food science , biochemistry , ecology

The use of dimethylsulfoxide as a solvent for synthetic purine and urea derivatives in the tobacco callus bioassay for cytokinin activity (10) has simplified the test procedure at two stages. First, the high solubility of these compounds in DMSO' has made it possible to test the less active materials, or those which are only slightly water-soluble, through a wide range of concentrations; secondly, full strength DMSO stock solutions act as sterilizing agents. Test compounds in DMSO solutions can be added directly to the cooling agar medium after autoclaving, thus avoiding destructive heating, and reducing the work, time, and equipment needed for the bioassay. In this laboratory DMSO was first employed as a solvent in the bioassay of diphenylurea. Bruce and Zwar (1) have reported the

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research