The Biosynthesis of Ethyl-β-glucoside in Extracts of Pea Seedlings | Zendy

Tin-Yin Liu | Zendy; Paul A. Castelfranco | Zendy

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The Biosynthesis of Ethyl-β-glucoside in Extracts of Pea Seedlings

Author(s) -

Tin-Yin Liu,

Paul A. Castelfranco

Publication year - 1970

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.45.4.424

Subject(s) - glucoside , enzyme , chemistry , epicotyl , beta (programming language) , enzyme assay , biochemistry , biosynthesis , stereochemistry , biology , botany , seedling , medicine , alternative medicine , pathology , computer science , programming language

An enzyme, which lacks cellobiase activity, responsible for the synthesis of ethyl-beta-glucoside has been found in the extracts of pea hooks (1-centimeter length of the apical portion of epicotyl) and has been partially purified by ammonium sulfate fractionation. The enzyme can transfer the glucosyl moiety from a group of phenolic beta-glucosides to ethanol. A specific beta-glucosyl donor, isosuccinimide beta-glucoside, isolated from the extracts of pea seedlings shows the highest activity. The characteristics of the enzyme which synthesizes ethyl-beta-glucoside and the glucosyl donor, isosuccinimide beta-glucoside, have been studied. The significance of this system (enzyme and isosuccinimide beta-glucoside) has been discussed.

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