Polyribosome Isolation in the Presence of Diethyl Pyrocarbonate | Zendy

Donald P. Weeks | Zendy; A Marcus | Zendy

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Polyribosome Isolation in the Presence of Diethyl Pyrocarbonate

Author(s) -

Donald P. Weeks,

A Marcus

Publication year - 1969

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.44.9.1291

Subject(s) - polysome , homogenization (climate) , reagent , chemistry , isolation (microbiology) , biochemistry , zea mays , in vitro , biology , ribosome , organic chemistry , agronomy , rna , microbiology and biotechnology , gene , biodiversity , ecology

Isolation of polyribosomes from wheat embryos and corn root tips in the presence of diethyl pyrocarbonate showed this reagent to have a protective effect on polyribosome structure. In addition, the use of diethyl pyrocarbonate allowed initial homogenization to be performed under less stringent conditions than those normally employed for polyribosome isolation. The use of the reagent is however limited, in that it is deleterious to in vitro ribosomal amino acid incorporation.

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