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Determination of Linkages in β-d-Glucans From Phaseolus aureus by exo-β-(1→3)-d-Glucanase
Author(s) -
K. K. Batra,
W. Z. Hassid
Publication year - 1969
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.44.5.755
Subject(s) - glucanase , phaseolus , glucan , chemistry , enzyme , alkali metal , hydrolysis , biochemistry , cellulose , polysaccharide , food science , chromatography , organic chemistry , botany , biology
An alkali-insoluble glucan synthesized from UDP-d-glucose by the particulate enzyme system from Phaseolus aureus is hydrolyzed by a highly purified exo-beta-(1 --> 3)-d-glucanase to d-glucose, to the extent of 91% in 24 hr. The alkali-insoluble glucan from GDP-d-glucose formed by the particulate enzyme system from the same plant which is known to be (from chemical data) a beta-(1 --> 4)-d-glucan (cellulose) is not acted upon by this glucanase.

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