Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations | Zendy

Stephen A. Bard | Zendy; Milton P. Gordon | Zendy

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Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations

Author(s) -

Stephen A. Bard,

Milton P. Gordon

Publication year - 1969

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.44.3.377

Subject(s) - chloroplast dna , chloroplast , dna , nuclear dna , spinach , ultracentrifuge , buoyant density , chemistry , extraction (chemistry) , biochemistry , composition (language) , biology , biophysics , chromatography , gene , mitochondrial dna , linguistics , philosophy

A method for the large-scale preparation of spinach chloroplasts using the Spinco Model L-4 zonal ultracentrifuge and for the extraction of DNA from the chloroplasts is described. Thirty-five percent of the chloroplast DNA (rho = 1.706 g/cc) differs from nuclear DNA (rho = 1.695 g/cc) in buoyant density. T(m), base composition, and renaturation properties. Sixty-five percent of the chloroplast DNA (rho = 1.696 g/cc) has the same buoyant density and T(m) as nuclear DNA, but it differs in base composition and renaturation properties.

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