Gas-Liquid Chromatography of Trimethylsilyl Derivatives of Abscisic Acid and Other Plant Hormones | Zendy

Larry A. Davis | Zendy; Daniel E. Heinz | Zendy; F. T. Addicott | Zendy

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Gas-Liquid Chromatography of Trimethylsilyl Derivatives of Abscisic Acid and Other Plant Hormones

Author(s) -

Larry A. Davis,

Daniel E. Heinz,

F. T. Addicott

Publication year - 1968

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.43.9.1389

Subject(s) - abscisic acid , chromatography , gas chromatography , trimethylsilyl , chemistry , acetone , gibberellin , elution , high performance liquid chromatography , organic chemistry , biochemistry , biology , botany , gene

This paper describes a new method for qualitative and quantitative assay of abscisic acid and other acidic plant hormones, such as indoleacetic acid and the gibberellins, by the gas-liquid chromatography of their trimethylsilyl derivatives. Interfering substances in plant extracts were largely removed by preliminary column chromatography with carbon-celite and elution of the abscisic acid with 60% acetone, permitting direct determination of abscisic acid by gas-liquid chromatography using a flame ionization detector. (A level of 0.65 mg/kg fr wt was found.) This method enables measurement of amounts of abscisic acid as low as 0.025 mug. In impure samples collected by gas-liquid chromatography the abscisic acid recovered could be measured quantitatively by use of its ultraviolet absorption maximum at 260 mmu.

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