Purification and Some Properties of the Nitrogenase From Soybean (Glycine max Merr.) Nodules | Zendy

Robert V. Klucas | Zendy; B. L. Koch | Zendy; Sterling A. Russell | Zendy; Harold J. Evans | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Purification and Some Properties of the Nitrogenase From Soybean (Glycine max Merr.) Nodules

Author(s) -

Robert V. Klucas,

B. L. Koch,

Sterling A. Russell,

Harold J. Evans

Publication year - 1968

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.43.12.1906

Subject(s) - nitrogenase , glycine , chemistry , chromatography , protamine sulfate , specific activity , precipitation , nitrogen , fraction (chemistry) , nitrogen fixation , biochemistry , enzyme , organic chemistry , protamine , amino acid , heparin , physics , meteorology

The nitrogenase system in cell-free extracts of soybean nodule bacteroids was fractionated into 2 components by use of protamine sulfate or polypropylene glycol precipitation followed by chromatography on DEAE-cellulose. Iron and molybdenum were concentrated in 1 fraction and iron in the other. Combination of fractions resulted in a striking stimulation in activity relative to the activity of individual fractions. The effect of different proportions of the 2 fractions on specific activities was studied. The ratios of the rates of reduction of acetylene and N(2) by extracts or fractions of different purities were relatively constant. Extracts or fractions retained most of their nitrogenase activities when stored in liquid N(2).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research