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Phosphorylation in Crested Wheatgrass Seeds at Low Water Potentials
Author(s) -
A. M. Wilson,
Grant A. Harris
Publication year - 1968
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.43.1.61
Subject(s) - phosphate , phosphorylation , sodium , chemistry , inorganic phosphate , adenosine triphosphate , nicotinamide adenine dinucleotide phosphate , hexose , adenosine diphosphate , potassium , biochemistry , botany , biology , enzyme , organic chemistry , platelet , platelet aggregation , oxidase test , immunology
Crested wheatgrass seeds [Agropyron desertorum (Fisch. ex Link) Schult.] were tested for their ability to carry on phosphorylation reactions at low water potentials. Seeds were treated with (32)P labeled sodium phosphate and incubated in air having different controlled relative humidities. Ion exchange chromatography and radioassay of phosphate esters indicated that some phosphorylation occurred at a water potential of -880 atmospheres. Seeds did not incorporate (32)P in nicotinamide adenine dinucleotide, adenosine triphosphate, and uridine diphosphate hexose until they were moistened to a water potential of -130 atmospheres.

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