In Vitro Autolysis of Plant Cell Walls

Primary cell walls of Zea mays prepared in a glycerol medium are capable of autolysis in vitro. Autolysis results in solubilization of about 10% of the wall substance during an 8 hour incubation period. Approximately 10% of the solubilized material is glucose and the remainder consists of an unidentified polymer which yields only glucose upon hydrolysis. Cell wall autolysis is a linear function of time of incubation and of wall concentration. The autolytic process occurs optimally over the pH range of 5.5 to 6.5. The possible relationship between autolytic capacity and capacity for elongation is discussed.