Relatedness Among Plants as Measured by the DNA-Agar Technique | Zendy

Arnold J. Bendich | Zendy; Ellis T. Bolton | Zendy

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Relatedness Among Plants as Measured by the DNA-Agar Technique

Author(s) -

Arnold J. Bendich,

Ellis T. Bolton

Publication year - 1967

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.42.7.959

Subject(s) - biology , dna , polynucleotide , homology (biology) , genetics , dna extraction , dna–dna hybridization , botany , polymerase chain reaction , gene

An improved method for extraction of plant DNA is described. Quantitative species comparisons based on DNA-DNA hybridization are reported for several members of the family Leguminosae and for barley, wheat and rye. A maximum of about 10% homology in DNA polynucleotide sequences is found between monocotyledons and dicotyledons tested, whereas 20 to 90% homology is observed within a family. Species compared using a DNA fraction enriched for redundant polynucleotide sequences generally appear to be more closely related than when whole DNA is used. DNA-DNA hybridization may be useful in systematic and evolutionary study of plants, and also as a possible screening procedure for interfertility of species.

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