Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-14C and Potential Intermediates | Zendy

Donald K. Dougall | Zendy; Michael M. Fulton | Zendy

Open Access

Biosynthesis of Protein Amino Acids in Plant Tissue Culture IV Isotope Competition Experiments using Glucose-U-¹⁴C and Potential Intermediates

Author(s) -

Donald K. Dougall,

Michael M. Fulton

Publication year - 1967

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.42.7.941

Subject(s) - biosynthesis , homoserine , amino acid , biochemistry , methionine , cystathionine beta synthase , threonine , amino acid synthesis , isotopes of carbon , protein biosynthesis , chemistry , serine , homocysteine , biology , organic chemistry , lysine , gene , enzyme , quorum sensing , virulence , total organic carbon

The isotope competition method with glucose-U-(14)C as a carbon source has been used to determine whether or not selected compounds contribute carbon to the biosynthesis of protein amino acids in cells derived from Paul's Scarlet Rose. Of 48 compounds tested, 15 contributed carbon to protein amino acids. The results show for the first time that homoserine is an intermediate in threonine biosynthesis; that homoserine, cystathionine and homocysteine are intermediates in methionine biosynthesis and that histidinol is an intermediate in histidine biosynthesis in plant cells.

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