Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings | Zendy

David L. Rayle | Zendy; William K. Purves | Zendy

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Isolation and Identification of Indole-3-Ethanol (Tryptophol) from Cucumber Seedlings

Author(s) -

David L. Rayle,

William K. Purves

Publication year - 1967

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.42.4.520

Subject(s) - cucumis , silicic acid , chemistry , chromatography , thin layer chromatography , indole test , size exclusion chromatography , hypocotyl , ethanol , mass spectrometry , biochemistry , botany , organic chemistry , biology , enzyme

Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thin layer and gas chromatography, and ultraviolet and visible spectroscopy, as well as by physiological characteristics.

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