Photosynthesis in Rhodospirillum rubrum. I. Autotrophic Carbon Dioxide Fixation
Author(s) -
Louise E. Anderson,
R. Clinton Fuller
Publication year - 1967
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.42.4.487
Subject(s) - rhodospirillum rubrum , carbon fixation , autotroph , photosynthesis , carbon dioxide , citric acid cycle , biochemistry , pentose phosphate pathway , ammonium , phosphate , metabolism , chemistry , facultative , botany , biology , bacteria , organic chemistry , glycolysis , enzyme , genetics
The incorporation and distribution of activity from (14)CO(2) was investigated under autotrophic conditions in the facultative photoautotroph, Rhodospirillum rubrum, with cells cultured on hydrogen, carbon dioxide, and ammonium sulfate. In 1 second (14)CO(2) fixation experiments essentially all of the activity was found in 3-phosphoglyceric acid: plotted against time percent incorporation into phosphate esters has a strikingly negative slope. These results suggest that under autotrophic conditions the reductive pentose phosphate cycle or the key reactions of the cycle play a major role in carbon metabolism in this photosynthetic bacterium. Incorporation into amino acids and into intermediates of the tricarboxylic acid cycle was quite low.
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