Preparation of Radioactive Gibberellin A1 and its Metabolism in Dwarf Peas | Zendy

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Preparation of Radioactive Gibberellin A₁ and its Metabolism in Dwarf Peas

Author(s) -

Hans Kende

Publication year - 1967

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.42.11.1612

Subject(s) - gibberellin , gibberellic acid , hydrolysis , chemistry , metabolism , fraction (chemistry) , macromolecule , biological activity , tritium , hydrogen , biochemistry , chromatography , organic chemistry , biology , botany , in vitro , germination , physics , nuclear physics

Gibberellin A(1)-3,4-(3)H was prepared by selective catalytic reduction of gibberellic acid with a mixture of tritium and hydrogen. (3)H-GA(1) was applied at physiological concentrations to dwarf peas and the metabolism of the hormone was investigated. (3)H-GA(1) was converted to an acidic, biologically active compound. Radioactive but biologically inactive compounds were also found in the neutral fraction and could not be converted to acidic gibberellins by hydrolysis. No attachment of gibberellin to any macromolecular fraction was evident.

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