Gibberellins of Sugarcane

In our hands a phosphate buffered celite column has given an adequate separation of GA(1) and GA(3). These 2 gibberellins are normally very difficult to separate. Young sugarcane growing under moisture stress contains at least 2 gibberellin-like substances. One is suspected to be GA(5). The other is unknown but has high activity in the barley endosperm assay and is neither GA(1) nor GA(3). Four-month-old cane contains 2 major growth promoters. From their chromatographic, fluorimetric and biological properties these are thought to be GA(1) and GA(3). Rapidly growing 6-month-old cane has a surprisingly low level of gibberellin-like substances.