Deamination of 4-Aminopyrimidine Nucleosides by Extracts of Rye Grass (Lolium perenne)
Author(s) -
David M. Frisch,
M. Charles
Publication year - 1966
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.41.3.475
Subject(s) - deamination , lolium perenne , chemistry , dactylis glomerata , absorbance , nucleoside , chromatography , biochemistry , agronomy , poaceae , biology , enzyme
A deaminase specific for 4-aminopyrimidine nucleosides has been found in rye grass. Lolium perenne. The pH optimum, temperature stability, Km values and specificity was determined.A new and more accurate method for following the course of deamination is reported. This method depends on the shift in maximum absorbance wavelength of the mixture containing the 4-aminopyrimidine nucleoside and its deaminated product.
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