Differentiation Between Indoleacetic Acid and the Citrus Auxin by Column Chromatography | Zendy

Rashad A. Khalifah | Zendy; Lowell N. Lewis | Zendy; Charles W. Coggins | Zendy

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Differentiation Between Indoleacetic Acid and the Citrus Auxin by Column Chromatography

Author(s) -

Rashad A. Khalifah,

Lowell N. Lewis,

Charles W. Coggins

Publication year - 1966

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.41.2.208

Subject(s) - auxin , avena , chromatography , chemistry , elution , column chromatography , gradient elution , biochemistry , high performance liquid chromatography , biology , botany , gene

A gradient elution column chromatography technique and a step-wise technique succeeded in differentiating between IAA and the citrus auxin. IAA was eluted ahead of the citrus auxin in both systems. The highest Avena curvature ever obtained from the citrus auxin occurred after the auxin had passed through the 2 purification techniques and a paper chromatography step. This is probably due to the elimination of inhibitors. Fluorometric assay, Ehrlich's reaction, thin-layer chromatography, and biological assay were used for the detection of IAA or citrus auxin in the column eluates.

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