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Electrophoretic and immunological properties of the soluble root protein complement of 6 Medicago sativa L. genotypes in the cold hardened and non-hardened physiological condition were compared. These 6 genotypes were chosen to represent a range of abilities to survive exposure to subfreezing temperatures when in the cold hardened condition.A zone of highly charged and/or low molecular weight protein components were found to be more prevalent in the protein complements of the cold-hardened material than the non-hardened material.Immunodiffusion plate tests were not so definitive as the electrophoretic patterns for identifying the genotypes or physiological conditions, but did corroborate the electrophoretic interpretations.

plant physiology

Electrophoretic and Immunological Comparisons of Soluble Root Proteins of <i>Medicago sativa</i> L. Genotypes in the Cold Hardened and Non-Hardened Condition

Electrophoretic and Immunological Comparisons of Soluble Root Proteins of Medicago sativa L. Genotypes in the Cold Hardened and Non-Hardened Condition