Electrophoretic and Immunological Comparisons of Soluble Root Proteins of Medicago sativa L. Genotypes in the Cold Hardened and Non-Hardened Condition | Zendy

Eugene Alfred Coleman | Zendy; R. J. Bula | Zendy; R. L. Davis | Zendy

Open Access

Electrophoretic and Immunological Comparisons of Soluble Root Proteins of Medicago sativa L. Genotypes in the Cold Hardened and Non-Hardened Condition

Author(s) -

Eugene Alfred Coleman,

R. J. Bula,

R. L. Davis

Publication year - 1966

Publication title -

plant physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.554

H-Index - 312

eISSN - 1532-2548

pISSN - 0032-0889

DOI - 10.1104/pp.41.10.1681

Subject(s) - electrophoresis , medicago sativa , radial immunodiffusion , biology , genotype , botany , horticulture , chemistry , biochemistry , immunology , gene , antibody

Electrophoretic and immunological properties of the soluble root protein complement of 6 Medicago sativa L. genotypes in the cold hardened and non-hardened physiological condition were compared. These 6 genotypes were chosen to represent a range of abilities to survive exposure to subfreezing temperatures when in the cold hardened condition.A zone of highly charged and/or low molecular weight protein components were found to be more prevalent in the protein complements of the cold-hardened material than the non-hardened material.Immunodiffusion plate tests were not so definitive as the electrophoretic patterns for identifying the genotypes or physiological conditions, but did corroborate the electrophoretic interpretations.

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